ARG82327
Rat PDGF AB ELISA Kit
Rat PDGF AB ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82327-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82327-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82327-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82327-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82327-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82327-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82327-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82327-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82327-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82327-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82327-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82327 Rat PDGF AB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat PDGF AB in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | PDGF AB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.8% Inter-Assay CV: 6.7% |
| Alternate Names | Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; PDGF-1; PDGF1; PDGF-A |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28576 Rat Platelet-derived growth factor subunit A |
|---|---|
| Gene Symbol | PDGFA |
| Gene Full Name | platelet-derived growth factor alpha polypeptide |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity). [UniProt] |
| Cellular Localization | Secreted. Note=Released by platelets upon wounding. [UniProt] |
| Highlight | Related products: PDGF antibodies; PDGF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82327 Rat PDGF AB ELISA Kit standard curve image
ARG82327 Rat PDGF AB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82327 Rat PDGF AB ELISA kit User manual |
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