ARG82325
Rat Laminin ELISA Kit
Rat Laminin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82325-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82325-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82325-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82325-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82325-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82325-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82325-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82325-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82325-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82325-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82325-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82325 Rat Laminin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Laminin in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | Laminin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.5% |
| Alternate Names | S-LAM-alpha; LAMA; Laminin-1 subunit alpha; PTBHS; Laminin-3 subunit alpha; S-LAM alpha; S-laminin subunit alpha; Laminin A chain; Laminin subunit alpha-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LAMA1 |
|---|---|
| Gene Full Name | laminin, alpha 1 |
| Background | This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014] |
| Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. [UniProt] |
| Highlight | Related products: Laminin antibodies; Laminin ELISA Kits; Laminin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82325 Rat Laminin ELISA Kit standard curve image
ARG82325 Rat Laminin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82325 Rat Laminin ELISA kit User manual |
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