ARG20712

Rat IgG2b Isotype Control antibody [KLH/G2b-1-2]

Rat IgG2b Isotype Control antibody [KLH/G2b-1-2] for ELISA,Flow cytometry,FLISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections

Overview

Product Description Rat Monoclonal antibody [KLH/G2b-1-2] as a negative control antibody for Rat IgG2b
Tested Application ELISA, FACS, FLISA, IHC-Fr, IHC-P
Specificity KLH
Host Rat
Clonality Monoclonal
Clone KLH/G2b-1-2
Isotype IgG2b, kappa
Target Name Rat IgG2b
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 1 µg/ml
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG20712 Rat IgG2b Isotype Control antibody [KLH/G2b-1-2] ELISA image

    ELISA: The plate was coated with serially diluted ARG20712 Rat IgG2b Isotype Control antibody [KLH/G2b-1-2]. Immunoglobulin was detected with ARG21696 Mouse anti-Rat IgG1 antibody [G17E7] (HRP), ARG21702 Mouse anti-Rat IgG2a antibody [2A8F4] (HRP), ARG21708 Mouse anti-Rat IgG2b antibody [2B10A8] (HRP), ARG21714 Mouse anti-Rat IgG2c antibody [2C8F1] (HRP), and ARG23836 Mouse anti-Rat IgM antibody [M2A1] (HRP) and quantified.

  • ARG20712 Rat IgG2b Isotype Control antibody [KLH/G2b-1-2] control image

    Control: Frozen section of Mouse spleen tissue stained with ARG20712 Rat IgG2b Isotype Control antibody [KLH/G2b-1-2] (left) and ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] (right), followed by ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) and DAPI.