ARG80248
Rat IgE ELISA Kit
Rat IgE ELISA Kit for ELISA and Rat
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80248-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80248-002 | Standard (Lyophilized) | 3 X 250 ng/vial | 4°C |
| ARG80248-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80248-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80248-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80248-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80248-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80248-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80248-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80248-010 | STOP solution | 12ml | 4°C |
| ARG80248-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80248 Rat IgE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IgE in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Rat IL-1,2,3,5,6,8,10, IFN, TNF；mouse IgE, human IgE |
| Target Name | IgE |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 4 ng/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 7.8 - 500 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Immunoglobulin E |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. |
|---|---|
| Highlight | Related products: IgE antibodies; IgE ELISA Kits; Related news: A designer cell-based histamine-specific human allergy profiler New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80248 Rat IgE ELISA Kit standard curve image
ARG80248 Rat IgE ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80248 Rat IgE ELISA Kit User's manual |
 Download
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