ARG80232
Rat IL4 ELISA Kit
Rat IL4 ELISA Kit for ELISA and Rat
Cancer kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80232-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80232-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80232-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80232-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80232-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80232-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80232-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80232-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80232-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80232-010 | STOP solution | 12ml | 4°C |
| ARG80232-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80232 Rat IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL4 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-1 sRI, IL-4, IL-7 R alpha, GDNF；mouse IL-4 |
| Target Name | IL4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il4 |
| Gene Full Name | interleukin 4 |
| Background | This protein is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Highlight | Related products: IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80232 Rat IL4 ELISA Kit standard curve image
ARG80232 Rat IL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80232 Rat IL4 ELISA Kit User's manual |
 Download
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