ARG81888
Rat IL3 ELISA Kit
Rat IL3 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81888-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81888-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81888-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81888-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81888-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81888-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81888-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81888-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81888-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81888-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81888-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81888 Rat IL3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL3 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 6.3% |
| Alternate Names | MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL3 |
|---|---|
| Gene Full Name | interleukin 3 |
| Background | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt] |
| Highlight | Related products: IL3 antibodies; IL3 ELISA Kits; IL3 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81888 Rat IL3 ELISA Kit standard curve image
ARG81888 Rat IL3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81888 Rat IL3 ELISA Kit User's manual |
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