ARG83008
Rat IL18 ELISA Kit
Rat IL18 ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG83008 Rat IL18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL18 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | IL18 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Interleukin-18 |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | IL18, Interleukin 18, IL1F4, IL 18, IGIF, IL 1g, Interleukin 18 (Interferon-Gamma-Inducing Factor), IFN Gamma Inducing Factor, Interleukin 1 Gamma, Interleukin 18, Iboctadekin, IL 1 Gamma, Interferon Gamma Inducing Factor, Interferon Gamma Inducing Factor |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL18 |
| Gene Full Name | Interleukin 18 |
| Background | The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2020] |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. [UniProt] |
| Cellular Localization | Cytoplasm, Secreted. [UniProt] |
| Highlight | Related products: IL18 antibodies; IL18 ELISA Kits; IL18 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83008 Rat IL18 ELISA Kit standard curve image
ARG83008 Rat IL18 ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83008 Rat IL18 ELISA Kit User manual |
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