ARG81692
Rat IL18 ELISA Kit
Rat IL18 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81692-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81692-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81692-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81692-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81692-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81692-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81692-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81692-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81692-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81692-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81692-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81692 Rat IL18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL18 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL18 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.4% |
| Alternate Names | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL18 |
| Gene Full Name | interleukin 18 |
| Background | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| Function | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
| Highlight | Related products: IL18 antibodies; IL18 ELISA Kits; IL18 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81692 Rat IL18 ELISA Kit standard curve image
ARG81692 Rat IL18 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81692 Rat IL18 ELISA Kit User's manual |
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