ARG80247
Rat IL17 / IL17A ELISA kit
Rat IL17 / IL17A ELISA kit for ELISA and Rat
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80247-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80247-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80247-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80247-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80247-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80247-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80247-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80247-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80247-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80247-010 | STOP solution | 12ml | 4°C |
| ARG80247-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80247 Rat IL17 / IL17A ELISA kit is an Enzyme Immunoassay kit for the quantification of Rat IL17 / IL17A in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Rat IL-1,2,3,4,5,6,7,8,9,10,11,12,13,17B, 17C, 17E, 17F, IFN- gamma, TNF, TGF, MCP-1, AR, CNTF, EGF, Epo, VEGF |
| Target Name | IL17 / IL17A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | IL-17A; Interleukin-17A; IL-17; CTLA8; IL17; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Il17a |
|---|---|
| Gene Full Name | interleukin 17A |
| Background | The originally described IL-17 protein, now known as IL-17A, is a disulfide linked homodimer, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. Recombinant murine IL-17A is a 30.0 kDa disulfide-linked homodimer of two 133 amino acid polypeptide chains. |
| Highlight | Related products: IL17 antibodies; IL17 ELISA Kits; IL17 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
| PTM | Found both in glycosylated and nonglycosylated forms. |
Images (1) Click the Picture to Zoom In
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ARG80247 Rat IL17 / IL17A ELISA kit standard curve image
ARG80247 Rat IL17 / IL17A ELISA kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80247 Rat IL17 / IL17A ELISA kit User's manual |
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