ARG81774
Rat IL13 ELISA Kit
Rat IL13 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81774-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81774-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81774-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81774-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81774-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81774-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81774-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81774-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81774-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81774-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81774-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81774 Rat IL13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL13 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL13 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 7.8% |
| Alternate Names | Interleukin-13; P600; IL-13 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL13 |
| Gene Full Name | interleukin 13 |
| Background | This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008] |
| Function | Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. [UniProt] |
| Highlight | Related products: IL13 antibodies; IL13 ELISA Kits; IL13 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81774 Rat IL13 ELISA Kit standard curve image
ARG81774 Rat IL13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81774 Rat IL13 ELISA Kit User's manual |
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