ARG82324
Rat IGF1 ELISA Kit
Rat IGF1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82324-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82324-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82324-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82324-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82324-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82324-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82324-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82324-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82324-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82324-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82324-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82324 Rat IGF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IGF1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | IGF1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.7% |
| Alternate Names | MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IGF1 |
| Gene Full Name | insulin-like growth factor 1 (somatomedin C) |
| Background | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015] |
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IGF1 antibodies; IGF1 ELISA Kits; IGF1 Duos / Panels; IGF1 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82324 Rat IGF1 ELISA Kit standard curve image
ARG82324 Rat IGF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82324 Rat IGF1 ELISA kit User manual |
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