ARG81879
Rat HGF ELISA Kit
Rat HGF ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81879-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81879-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81879-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81879-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81879-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81879-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81879-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81879-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81879-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81879-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81879-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81879 Rat HGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat HGF in serum, plasma (EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0%; Inter-Assay CV: 8.0% |
| Alternate Names | HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HGF |
| Gene Full Name | hepatocyte growth factor (hepapoietin A; scatter factor) |
| Background | Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt] |
| Highlight | Related products: HGF antibodies; HGF ELISA Kits; HGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81879 Rat HGF ELISA Kit standard curve image
ARG81879 Rat HGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81879 Rat HGF ELISA Kit User manual |
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