ARG82852
Rat H-FABP / Cardiac FABP ELISA Kit
Rat H-FABP / Cardiac FABP ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82852-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82852-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82852-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82852-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82852-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82852-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82852-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82852-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82852-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82852-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82852-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82852 Rat H-FABP / Cardiac FABP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat H-FABP / Cardiac FABP in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | H-FABP / Cardiac FABP |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.9 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 6.6% |
| Alternate Names | FABP11; H-FABP; O-FABP; Heart-type fatty acid-binding protein; MDGI; Fatty acid-binding protein 3; Muscle fatty acid-binding protein; Mammary-derived growth inhibitor; Fatty acid-binding protein, heart; M-FABP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FABP3 |
| Gene Full Name | fatty acid binding protein 3, muscle and heart |
| Background | The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| Function | FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82852 Rat H-FABP / Cardiac FABP ELISA Kit standard curve image
ARG82852 Rat H-FABP / Cardiac FABP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82852 Rat H-FABP / Cardiac FABP ELISA Kit User manual |
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