ARG82552
Rat GDNF Receptor alpha 1 ELISA Kit
Rat GDNF Receptor alpha 1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82552-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82552-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82552-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82552-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82552-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82552-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82552-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82552-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82552-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82552-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82552-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82552 Rat GDNF Receptor alpha 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat GDNF Receptor alpha 1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | GDNF Receptor alpha 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 7.1% |
| Alternate Names | GDNFR-alpha-1; GDNFRA; GFR-ALPHA-1; RET ligand 1; GFR-alpha-1; RET1L; GDNF receptor alpha-1; TRNR1; TGF-beta-related neurotrophic factor receptor 1; RETL1; GDNFR; GDNF family receptor alpha-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFRA1 |
| Gene Full Name | GDNF family receptor alpha 1 |
| Background | Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009] |
| Function | Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Highlight | Related products: GDNF Receptor antibodies; GDNF Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82552 Rat GDNF Receptor alpha 1 ELISA Kit standard curve image
ARG82552 Rat GDNF Receptor alpha 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82552 Rat GDNF Receptor alpha 1 ELISA Kit User manual |
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