ARG81648
Rat GDNF ELISA Kit
Rat GDNF ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81648-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81648-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81648-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81648-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81648-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81648-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81648-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81648-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81648-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81648-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81648-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81648 Rat GDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat GDNF in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | GDNF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.6%; Inter-Assay CV: 7.7% |
| Alternate Names | ATF; Astrocyte-derived trophic factor; HSCR3; hGDNF; Glial cell line-derived neurotrophic factor; HFB1-GDNF; ATF1; ATF2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q07731 Rat Glial cell line-derived neurotrophic factor |
|---|---|
| Gene Symbol | GDNF |
| Gene Full Name | glial cell derived neurotrophic factor |
| Background | This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010] |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. [UniProt] |
| Highlight | Related products: GDNF antibodies; GDNF ELISA Kits; GDNF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81648 Rat GDNF ELISA Kit standard curve image
ARG81648 Rat GDNF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81648 Rat GDNF ELISA Kit User's manual |
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