ARG82100
Rat FGFBP1 ELISA Kit
Rat FGFBP1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82100-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82100-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82100-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82100-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82100-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82100-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82100-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82100-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82100-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82100-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82100-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82100 Rat FGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGFBP1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FGFBP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.2% |
| Alternate Names | Fibroblast growth factor-binding protein 1; 17 kDa heparin-binding growth factor-binding protein; FGF-BP; FGF-binding protein 1; HBp17; FGFBP; 17 kDa HBGF-binding protein; FGFBP-1; HBP17; FGF-BP1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QY10 Rat Fibroblast growth factor-binding protein 1 |
|---|---|
| Gene Symbol | FGFBP1 |
| Gene Full Name | fibroblast growth factor binding protein 1 |
| Background | This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011] |
| Function | Acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth. [UniProt] |
| Cellular Localization | Secreted, extracellular space. Cell membrane; Peripheral membrane protein. Note=Extracellular and plasma membrane-associated. Colocalizes with HSPG2 in the pericellular environment of squamous cell carcinomas. [UniProt] |
| Highlight | Related products: FGFBP1 antibodies; FGFBP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82100 Rat FGFBP1 ELISA Kit standard curve image
ARG82100 Rat FGFBP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82100 Rat FGFBP1 ELISA Kit User's manual |
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