ARG82105
Rat FGF23 ELISA Kit
Rat FGF23 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82105-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82105-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82105-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82105-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82105-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82105-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82105-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82105-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82105-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82105-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82105-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82105 Rat FGF23 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGF23 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FGF23 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9%; Inter-Assay CV: 6.5% |
| Alternate Names | ADHR; Phosphatonin; HPDR2; FGFN; Tumor-derived hypophosphatemia-inducing factor; PHPTC; FGF-23; Fibroblast growth factor 23; HYPF |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF23 |
| Gene Full Name | fibroblast growth factor 23 |
| Background | This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013] |
| Function | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. [UniProt] |
| Cellular Localization | Secreted. Note=Secretion is dependent on O-glycosylation. [UniProt] |
| Highlight | Related products: FGF23 antibodies; FGF23 ELISA Kits; FGF23 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82105 Rat FGF23 ELISA Kit standard curve image
ARG82105 Rat FGF23 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82105 Rat FGF23 ELISA kit User manual |
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