ARG82107
Rat FGF21 ELISA Kit
Rat FGF21 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82107-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82107-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82107-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82107-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82107-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82107-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82107-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82107-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82107-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82107-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82107-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82107 Rat FGF21 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGF21 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FGF21 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.2% |
| Alternate Names | Fibroblast growth factor 21; FGF-21 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF21 |
|---|---|
| Gene Full Name | fibroblast growth factor 21 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008] |
| Function | Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: FGF21 antibodies; FGF21 ELISA Kits; FGF21 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82107 Rat FGF21 ELISA Kit standard curve image
ARG82107 Rat FGF21 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82107 Rat FGF21 ELISA Kit User's manual |
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