ARG81925
Rat Eotaxin ELISA Kit
Rat Eotaxin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81925-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81925-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81925-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81925-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81925-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81925-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81925-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81925-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81925-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81925-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81925-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81925 Rat Eotaxin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Eotaxin in serum, plasma (EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Eotaxin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 5.1% |
| Alternate Names | SCYA11; C-C motif chemokine 11; Eosinophil chemotactic protein; Eotaxin; Small-inducible cytokine A11 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL11 |
| Gene Full Name | chemokine (C-C motif) ligand 11 |
| Background | This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014] |
| Function | In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. [UniProt] |
| Highlight | Related products: Eotaxin antibodies; Eotaxin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-linked glycan consists of a Gal-GalNAc disaccharide which is modified with up to 2 sialic acid residues. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81925 Rat Eotaxin ELISA Kit standard curve image
ARG81925 Rat Eotaxin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81925 Rat Eotaxin ELISA Kit User's manual |
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