ARG82336
Rat Endothelin 1 ELISA Kit
Rat Endothelin 1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82336-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82336-002 | Standard | 2 X 1 ng/vial | 4°C |
| ARG82336-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82336-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82336-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82336-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82336-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82336-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82336-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82336-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82336-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82336 Rat Endothelin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Endothelin 1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | Endothelin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 1.95 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 3.9 - 250 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.3% |
| Alternate Names | HDLCQ7; ET1; PPET1; ET-1; Preproendothelin-1; ARCND3; Endothelin-1; QME |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EDN1 |
| Gene Full Name | endothelin 1 |
| Background | The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009] |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Endothelin antibodies; Endothelin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82336 Rat Endothelin 1 ELISA Kit standard curve image
ARG82336 Rat Endothelin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82336 Rat Endothelin 1 ELISA Kit User manual |
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