ARG81897
Rat EPO ELISA Kit
Rat EPO ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81897-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81897-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81897-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81897-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81897-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81897-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81897-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81897-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81897-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81897-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81897-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81897 Rat EPO ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat EPO in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | EPO |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 7.2% |
| Alternate Names | MVCD2; EP; Epoetin; Erythropoietin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EPO |
| Gene Full Name | erythropoietin |
| Background | This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008] |
| Function | Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. [UniProt] |
| Highlight | Related products: EPO antibodies; EPO ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG81897 Rat EPO ELISA Kit standard curve image
ARG81897 Rat EPO ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81897 Rat EPO ELISA Kit User's manual |
 Download
|
