ARG81992
Rat CXCL4 / PF4 ELISA Kit
Rat CXCL4 / PF4 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81992-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81992-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81992-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81992-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81992-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81992-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81992-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81992-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81992-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81992-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81992-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81992 Rat CXCL4 / PF4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXCL4 / PF4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CXCL4 / PF4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4%; Inter-Assay CV: 6.1% |
| Alternate Names | Platelet factor 4; Iroplact; CXCL4; C-X-C motif chemokine 4; Oncostatin-A; SCYB4; PF-4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PF4 |
| Gene Full Name | platelet factor 4 |
| Background | This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014] |
| Function | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. [UniProt] |
| Highlight | Related products: CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81992 Rat CXCL4 / PF4 ELISA Kit standard curve image
ARG81992 Rat CXCL4 / PF4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81992 Rat CXCL4 / PF4 ELISA Kit User manual |
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