ARG81908
Rat CXCL3 / GRO gamma ELISA Kit
Rat CXCL3 / GRO gamma ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81908-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81908-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81908-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81908-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81908-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81908-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81908-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81908-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81908-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81908-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81908-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81908 Rat CXCL3 / GRO gamma ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXCL3 / GRO gamma in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CXCL3 / GRO gamma |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.6%; Inter-Assay CV: 7.4% |
| Alternate Names | Macrophage inflammatory protein 2-beta; MIP2-beta; SCYB3; GRO-gamma; Growth-regulated protein gamma; 5-73; GROg; 1-73; MIP2B; C-X-C motif chemokine 3; CINC-2b; GRO3; MIP-2b |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CXCL3 |
| Gene Full Name | chemokine (C-X-C motif) ligand 3 |
| Background | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014] |
| Function | Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. [UniProt] |
| Highlight | Related products: CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81908 Rat CXCL3 / GRO gamma ELISA Kit standard curve image
ARG81908 Rat CXCL3 / GRO gamma ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81908 Rat CXCL3 / GRO gamma ELISA Kit User's manual |
 Download
|
