ARG81809
Rat CST3 / Cystatin C ELISA Kit
Rat CST3 / Cystatin C ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81809-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81809-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81809-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81809-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81809-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81809-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81809-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81809-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81809-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81809-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81809-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81809 Rat CST3 / Cystatin C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CST3 / Cystatin C in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CST3 / Cystatin C |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9%; Inter-Assay CV: 5.8% |
| Alternate Names | Cystatin-C; Neuroendocrine basic polypeptide; Post-gamma-globulin; ARMD11; Cystatin-3; Gamma-trace |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CST3 |
| Gene Full Name | cystatin C |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014] |
| Function | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. [UniProt] |
| Highlight | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81809 Rat CST3 / Cystatin C ELISA Kit standard curve image
ARG81809 Rat CST3 / Cystatin C ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81809 Rat CST3 / Cystatin C ELISA Kit User's manual |
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