ARG82503
Rat CNTN1 / Contactin 1 ELISA Kit
Rat CNTN1 / Contactin 1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82503-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82503-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82503-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82503-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82503-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82503-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82503-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82503-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82503-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82503-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82503-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82503 Rat CNTN1 / Contactin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CNTN1 / Contactin 1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | CNTN1 / Contactin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 6.8% |
| Alternate Names | Contactin-1; F3; Glycoprotein gp135; GP135; Neural cell surface protein F3; MYPCN |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CNTN1 |
| Gene Full Name | contactin 1 |
| Background | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt] |
| Highlight | Related products: Contactin antibodies; Contactin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82503 Rat CNTN1 / Contactin 1 ELISA Kit standard curve image
ARG82503 Rat CNTN1 / Contactin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82503 Rat CNTN1 / Contactin 1 ELISA Kit User manual |
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