ARG82079
Rat CNTF ELISA Kit
Rat CNTF ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82079-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82079-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82079-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82079-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82079-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82079-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82079-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82079-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82079-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82079-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82079-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82079 Rat CNTF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CNTF in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CNTF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4%; Inter-Assay CV: 7.4% |
| Alternate Names | HCNTF; CNTF; Ciliary neurotrophic factor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CNTF |
| Gene Full Name | ciliary neurotrophic factor |
| Background | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: CNTF antibodies; CNTF ELISA Kits; CNTF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82079 Rat CNTF ELISA Kit standard curve image
ARG82079 Rat CNTF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82079 Rat CNTF ELISA Kit User's manual |
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