ARG81722
Rat CD86 ELISA Kit
Rat CD86 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81722-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81722-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81722-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81722-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81722-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81722-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81722-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81722-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81722-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81722-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81722-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81722 Rat CD86 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD86 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD86 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 7.2% |
| Alternate Names | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD86 |
|---|---|
| Gene Full Name | CD86 molecule |
| Background | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] |
| Highlight | Related products: CD86 antibodies; CD86 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81722 Rat CD86 ELISA Kit standard curve image
ARG81722 Rat CD86 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81722 Rat CD86 ELISA Kit User's manual |
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