ARG81781
Rat CD62E / E-Selectin ELISA Kit
Rat CD62E / E-Selectin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81781-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81781-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81781-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81781-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81781-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81781-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81781-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81781-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81781-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81781-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81781-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81781 Rat CD62E / E-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat soluble CD62E / E-Selectin in serum, plasma (heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD62E / E-Selectin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.2% |
| Alternate Names | E-selectin; CD62E; ELAM-1; ELAM1; CD antigen CD62E; ELAM; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; ESEL; LECAM2; CD62 antigen-like family member E |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SELE |
| Gene Full Name | selectin E |
| Background | The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008] |
| Function | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. [UniProt] |
| Highlight | Related products: CD62E antibodies; CD62E ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81781 Rat CD62E / E-Selectin ELISA Kit standard curve image
ARG81781 Rat CD62E / E-Selectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81781 Rat sCD62E / sE-Selectin ELISA Kit User manual |
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