ARG81832
Rat CD255 / TWEAK ELISA Kit
Rat CD255 / TWEAK ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81832-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81832-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81832-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81832-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81832-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81832-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81832-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81832-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81832-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81832-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81832-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81832 Rat CD255 / TWEAK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD255 / TWEAK in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD255 / TWEAK |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.3% |
| Alternate Names | APO3L; DR3LG; TWEAK; TNLG4A |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFSF12 |
|---|---|
| Gene Full Name | TNF superfamily member 12 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010] |
| Function | Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. [UniProt] |
| Cellular Localization | Plasma membrane; Extracellular region or secreted. [UniProt] |
| Highlight | Related products: CD255 antibodies; CD255 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81832 Rat CD255 / TWEAK ELISA Kit standard curve image
ARG81832 Rat CD255 / TWEAK ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81832 Rat TWEAK ELISA Kit User's manual |
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