ARG81128

Rat Atrial Natriuretic Peptide (ANP) ELISA Kit

Rat Atrial Natriuretic Peptide (ANP) ELISA Kit for ELISA and Rat

Overview

Product Description

ARG81128 Rat Atrial Natriuretic Peptide (ANP) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Atrial Natriuretic Peptide in serum, plasma, cell culture and tissue extract.

Tested Reactivity Rat
Tested Application ELISA
Target Name ANP / Atrial Natriuretic Peptide
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 6.7 pg/ml
Sample Type Serum, plasma, cell culture and tissue extract
Standard Range 7.8 - 2000 pg/ml
Sample Volume 50 µl
Alternate Names Natriuretic peptides A; ANF; Atriopeptin II; RATANF; Prepronatriodilatin; ANP; Atrial natriuretic peptide; Atriopeptin III; Pnd; Atriopeptin I

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24602 Rat NPPA

Swiss-port # P01161 Rat Natriuretic peptides A

Gene Symbol Nppa
Gene Full Name natriuretic peptide A
Background The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]
Function Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity). [UniProt]
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PTM Cleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

Images (1) Click the Picture to Zoom In

  • ARG81128 Rat Atrial Natriuretic Peptide (ANP) ELISA Kit standard curve image

    ARG81128 Rat Atrial Natriuretic Peptide (ANP) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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