ARG82626
Rat Apolipoprotein B ELISA Kit
Rat Apolipoprotein B ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG82626 Rat Apolipoprotein B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Apolipoprotein B in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | The following recombinant Rat proteins were tested and exhibited no cross-reactivity or interference: ApoAI, BMP1, BMP2, BMP3, BMP4, BMP7, CRP, HGF, HSP27, IL1 alpha, IL1 RI, IL1 beta, IL1RA, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, sIL2R, sIL6R, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| Target Name | Apolipoprotein B |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 6.3 ng/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 12.5 - 800 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | Apolipoprotein B-100; apoB-48; Apo B-100; apoB-100; Apo B-48; FLDB; LDLCQ4 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | APOB |
| Gene Full Name | apolipoprotein B |
| Background | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008] |
| Function | Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. [UniProt] |
| Cellular Localization | Cytoplasm. Secreted. [UniProt] |
| Highlight | Related products: Apolipoprotein antibodies; Apolipoprotein ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82626 Rat Apolipoprotein B ELISA Kit standard curve image
ARG82626 Rat Apolipoprotein B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82626 Rat Apolipoprotein B ELISA Kit User manual |
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