ARG81984
Rat ApoJ / Clusterin ELISA Kit
Rat ApoJ / Clusterin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81984-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81984-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81984-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81984-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81984-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81984-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81984-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81984-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81984-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81984-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81984-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81984 Rat ApoJ / Clusterin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat ApoJ / Clusterin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ApoJ / Clusterin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.1% |
| Alternate Names | Clusterin; Apolipoprotein J; SGP-2; SP-40; APOJ; ApoJbeta; CLU2; CLU1; NA1/NA2; TRPM2; CLI; SGP2; Apo-J; TRPM-2; ApoJalpha; Aging-associated gene 4 protein; APO-J; Complement cytolysis inhibitor a chain; Ku70-binding protein 1; Complement cytolysis inhibitor b chain; Testosterone-repressed prostate message 2; Complement-associated protein SP-40,40; KUB1; AAG4; Complement cytolysis inhibitor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLU |
| Gene Full Name | clusterin |
| Background | The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011] |
| Function | Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. [UniProt] |
| Highlight | Related products: ApoJ antibodies; ApoJ ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation. Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81984 Rat ApoJ / Clusterin ELISA Kit standard curve image
ARG81984 Rat ApoJ / Clusterin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81984 Rat ApoJ / Clusterin ELISA Kit User's manual |
 Download
|
