ARG81967
Rat Angiopoietin 2 ELISA Kit
Rat Angiopoietin 2 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81967-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81967-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81967-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81967-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81967-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81967-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81967-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81967-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81967-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81967-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81967-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81967 Rat Angiopoietin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Angiopoietin 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Angiopoietin 2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.0% |
| Alternate Names | ANG-2; Angiopoietin-2; ANG2; AGPT2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANGPT2 |
| Gene Full Name | angiopoietin 2 |
| Background | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. [UniProt] |
| Highlight | Related products: Angiopoietin antibodies; Angiopoietin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81967 Rat Angiopoietin 2 ELISA Kit standard curve image
ARG81967 Rat Angiopoietin 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81967 Rat Angiopoietin 2 ELISA Kit User manual |
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