ARG82288
Porcine TGF beta 1 ELISA Kit
Porcine TGF beta 1 ELISA Kit for ELISA and Pig
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82288-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82288-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82288-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82288-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82288-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82288-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82288-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82288-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82288-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82288-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82288-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82288 Porcine TGF beta 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine TGF beta 1 in serum, plasma (EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Cross-reactivity: TGF beta 2, 3 and 5: <1% |
| Target Name | TGF beta 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (EDTA), urine and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 7.5% |
| Alternate Names | TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TGFB1 |
| Gene Full Name | transforming growth factor, beta 1 |
| Background | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009] |
| Function | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: TGF beta antibodies; TGF beta ELISA Kits; TGF beta recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82288 Porcine TGF beta 1 ELISA Kit standard curve image
ARG82288 Porcine TGF beta 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82288 Porcine TGF beta 1 ELISA kit User manual |
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