ARG82638
Porcine PDGF (total) ELISA Kit
Porcine PDGF (total) ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG82638 Porcine PDGF (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine PDGF (total) in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | This kit reacts to PDGF-A, PDGF-B, PDGF-C, PDGF-D, PDGF-AA, PDGF-AB and PDGF-BB proteins. The following recombinant Porcine proteins were tested and exhibited no cross-reactivity or interference: ApoAI, BMP1, BMP2, BMP3, BMP4, HGF, HSP27, IFN gamma, IL1 beta, IL1RA, IL2, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, IL23, MMP2, MMP9, sIL2R, sIL6R, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| Target Name | PDGF (total) |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 23.5 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 47 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4% Inter-Assay CV: 9% |
| Alternate Names | PDGFA: Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; PDGF-1; PDGF1; PDGF-A PDGFB: SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | PDGFA; PDGFB |
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| Gene Full Name | platelet-derived growth factor alpha polypeptide platelet-derived growth factor beta polypeptide |
| Background | PDGFA: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] PDGFB: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Function | PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. [UniProt] PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. [UniProt] |
| Cellular Localization | Secreted. Note=Released by platelets upon wounding. [UniProt] |
| Highlight | Related products: PDGF antibodies; PDGF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82638 Porcine PDGF (total) ELISA Kit standard curve image
ARG82638 Porcine PDGF (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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| ARG82638 Porcine PDGF (total) ELISA kit User manual |
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