ARG82274
Porcine Insulin ELISA Kit
Porcine Insulin ELISA Kit for ELISA and Pig
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82274-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82274-002 | Standard (Lyophilized) | 2 X 0.5 pmol/vial | 4°C |
| ARG82274-003 | Standard/Sample diluent | 20 ml | 4°C |
| ARG82274-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG82274-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG82274-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG82274-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG82274-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG82274-009 | TMB substrate | 12 ml | 4°C (Protect from light) |
| ARG82274-010 | STOP solution | 12 ml | 4°C |
| ARG82274-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82274 Porcine Insulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine Insulin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Cross-react with Human and Dog Insulin. Not react with Human C-Peptide and IGF-I. |
| Target Name | Insulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pmol/L |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pmol/L |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
Application Instructions
| Assay Time | ~ 3.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INS |
| Gene Full Name | insulin |
| Background | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82274 Porcine Insulin ELISA Kit standard curve image
ARG82274 Porcine Insulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82274 Porcine Insulin ELISA kit User's manual |
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