ARG83025
Porcine IL4 ELISA Kit
Porcine IL4 ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG83025 Porcine IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine IL4 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Cross-reacts with: Human IL4. Felinee IL4. Not cross-reacts with: Mouse IL4. Rat IL4. Canine IL4. |
| Target Name | IL4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Interleukin-4 |
| Sensitivity | 0.312 ng/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 0.625 - 40 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | IL4, Interleukin 4, IL 4, Lymphocyte Stimulatory Factor 1, BCGF 1, BCGF1, BSF1, B cell Stimulatory Factor 1, B Cell Growth Factor 1, Interleukin 4, Binetrakin, Pitrakinra, MGC79402, BSF 1, B Cell Stimulatory Factor 1 |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | IL4 |
| Gene Full Name | Interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020] |
| Function | Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 . [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Disulfide bond, Glycoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83025 Porcine IL4 ELISA Kit standard curve image
ARG83025 Porcine IL4 ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83025 Porcine IL4 ELISA Kit User manual |
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