ARG83026
Porcine IL10 ELISA Kit
Porcine IL10 ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG83026 Porcine IL10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine IL10 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human IL-4, porcine, IFN gamma. Mouse IL-4, porcine, IFN gamma. Rat IL-4, porcine, IFN gamma. |
| Target Name | IL10 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Interleukin 10 |
| Sensitivity | 23.44 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 46.875 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | IL10, Interleukin 10 ,IL 10, CSIF, Cytokine Synthesis Inhibitory Factor, IL10A, TGIF, T Cell Growth Inhibitory Factor, Interleukin 10, GVHDS |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | IL10 |
| Gene Full Name | Interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL10 antibodies; IL10 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Disulfide bond, Glycoprotein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83026 Porcine IL10 ELISA Kit standard curve image
ARG83026 Porcine IL10 ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83026 Porcine IL10 ELISA Kit User manual |
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