ARG82302
Porcine IL1 beta ELISA Kit
Porcine IL1 beta ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG82302 Porcine IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine IL1 beta in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Not react with Porcine IL1 alpha, IL1RA and Human IL1 beta. |
| Target Name | IL1 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 9% Inter-Assay CV: < 9% |
| Alternate Names | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL1B |
|---|---|
| Gene Full Name | interleukin 1, beta |
| Background | IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| Function | IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Lysosome. Secreted, exosome. Secreted. [UniProt] |
| Highlight | Related products: IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82302 Porcine IL1 beta ELISA Kit standard curve image
ARG82302 Porcine IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82302 Porcine IL1 beta ELISA kit User's manual |
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