ARG30043
Phospho ZAP70 Antibody Duo (pY319, pY493)
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51657 | anti-ZAP70 phospho (Tyr319) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, WB | 50 μl |
| ARG51658 | anti-ZAP70 phospho (Tyr493) antibody | Rabbit pAb | Hu, Ms, Rat | IHC-P, WB | 50 μl |
Overview
| Product Description | ZAP70, Zeta-chain-associated protein kinase 70 (70kDa), is a tyrosine kinase protein normally expressed near the surface membrane of T cells and natural killer cells and plays a critical role in the events involved in initiating T-cell responses by the antigen receptor. Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. ARG30043 ZAP70 phospho Duos (pY319, pY493), includeing antibodies react two important phosphorylation site of ZAP70 on Tyr-319 and Tyr-493, is useful for ZAP70 signaling transduction studies. |
|---|---|
| Target Name | ZAP70 |
| Alternate Names | Phospho ZAP70 antibody; Phospho Zeta chain of T cell receptor associated protein kinase 70 antibody; ZAP70 phospho (Tyr319) antibody; ZAP70 phospho (Tyr493) antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Phospho Zeta chain of T cell receptor associated protein kinase 70 (ZAP70) Antibody Duo (pY319, pY493) |
|---|---|
| Research Area | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody |
Images (5) Click the Picture to Zoom In
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody WB image
Western Blot: extracts from Jurkat cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody WB image
Western Blot: extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
-
ARG51658 anti-Zap-70 phospho (Tyr493) antibody WB image
Western Blot: extracts from JK cells untreated or treated with anisomycin stained with anti-Zap-70 (phospho Tyr493) antibody ARG51658.
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
-
ARG51658 anti-Zap-70 phospho (Tyr493) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Zap-70 (phospho Tyr493) antibody ARG51658 (left) or the same antibody preincubated with blocking peptide (right).
