ARG83413

Nitrite reductase Assay Kit

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96 wells

419.00

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Datasheet
Protocols

Overview

Product Description	ARG83413 Nitrite reductase Assay Kit can be used to measure Nitrite reductase in tissue extracts, cell lysate, cell culture media and other biological fluids.
Tested Reactivity	Other
Tested Application	FuncSt
Target Name	Nitrite reductase
Conjugation Note	Read at 540 nm
Sensitivity	0.01 μmol/ml
Sample Type	Tissue extracts, cell lysate, cell culture media and other biological fluids.
Standard Range	0.02 μmol/ml - 2 μmol/ml
Sample Volume	20 μl
Alternate Names	Nitrite reductase

Application Instructions

Assay Time	30 min

Properties

Form	96 well
Storage Instruction	Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background	Nitrite reductase (NIR) has been determined to be the major NO producer in mammalian cells under hypoxic conditions, such as those that commonly occur in hypoxic vasodilation of cardiovascular systems, muscle tissue during extensive exercise, and ischemic tissues. Unlike NOS, NIR produces NO in an O2-independent manner, suggesting the potential contribution of NO in mediating hypoxic signalling-dependent cellular responses. Haem-binding proteins, such as haemoglobin and myoglobin, and cytochrome c, cytochrome bc1, and cytochrome c oxidase of the mitochondrial electron transport chain, have been reported to generate NO via NIR-dependent catalysis.

Background

Nitrite reductase (NIR) has been determined to be the major NO producer in mammalian cells under hypoxic conditions, such as those that commonly occur in hypoxic vasodilation of cardiovascular systems, muscle tissue during extensive exercise, and ischemic tissues. Unlike NOS, NIR produces NO in an O2-independent manner, suggesting the potential contribution of NO in mediating hypoxic signalling-dependent cellular responses. Haem-binding proteins, such as haemoglobin and myoglobin, and cytochrome c, cytochrome bc1, and cytochrome c oxidase of the mitochondrial electron transport chain, have been reported to generate NO via NIR-dependent catalysis.