ARG81188
Nitric Oxide Synthase Assay Kit
Nitric Oxide Synthase Assay Kit for Functional study and Human,Mouse,Rat,Mammal
Overview
| Product Description | ARG81188 Nitric Oxide Synthase Assay Kit is a detection kit for the quantification of Nitric Oxide Synthase activity in serum, plasma, whole blood, cell culture media, tissue or cell lysates. |
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| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | FuncSt |
| Target Name | Nitric Oxide Synthase |
| Conjugation | Un-conjugated |
| Sensitivity | 0.25 U/L |
| Sample Type | Serum, plasma, whole blood, cell culture media, tissue or cell lysates. |
| Standard Range | 0.25 - 25 U/L |
| Sample Volume | 20 µl |
| Alternate Names | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | ~ 2 hour |
Properties
| Form | Liquid |
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| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | NOS3 |
| Gene Full Name | nitric oxide synthase 3 (endothelial cell) |
| Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| PTM | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Title | Download Link |
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| ARG81188 Nitric Oxide Synthase Assay Kit User's manual |
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