ARG81676
Mouse sTNFR2 ELISA Kit
Mouse sTNFR2 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81676-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81676-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81676-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81676-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81676-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81676-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81676-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81676-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81676-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81676-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81676-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81676 Mouse sTNFR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse sTNFR2 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | sTNFR2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.9%; Inter-Assay CV: 7.7% |
| Alternate Names | TNFR1B; CD antigen CD120b; p75; CD120b; p75TNFR; TNF-R-II; TNFBR; TBPII; TNFR80; TBP-2; TNFR2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; Etanercept; Tumor necrosis factor receptor superfamily member 1B; TNFR-II; TNF-R75; TNF-R2; TNF-RII; p80 TNF-alpha receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25119 Mouse Tumor necrosis factor receptor superfamily member 1B |
|---|---|
| Gene Symbol | TNFRSF1B |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 1B |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008] |
| Function | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. [UniProt] |
| Highlight | Related products: TNFR2 antibodies; TNFR2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated; mainly on serine residues and with a very low level on threonine residues. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81676 Mouse sTNFR2 ELISA Kit standard curve image
ARG81676 Mouse sTNFR2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81676 Mouse sTNFR2 ELISA Kit User's manual |
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