ARG81663
Mouse beta NGF ELISA Kit
Mouse beta NGF ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81663-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81663-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81663-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81663-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81663-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81663-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81663-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81663-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81663-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81663-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81663-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81663 Mouse beta NGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse beta NGF in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4. |
| Target Name | beta NGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.2%; Inter-Assay CV: 7.4% |
| Alternate Names | HSAN5; Beta-nerve growth factor; Beta-NGF; NGFB |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NGF |
| Gene Full Name | nerve growth factor (beta polypeptide) |
| Background | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. [UniProt] |
| Highlight | Related products: beta NGF antibodies; beta NGF ELISA Kits; beta NGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81663 Mouse beta NGF ELISA Kit standard curve image
ARG81663 Mouse beta NGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81663 Mouse NGF ELISA Kit User manual |
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