ARG21706

Mouse anti-Rat IgG2b antibody [2B10A8] (FITC)

Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) for Flow cytometry,FLISA,IHC-Frozen sections,Western blot and Rat

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [2B10A8] recognizes Rat IgG2b
Tested Reactivity Rat
Tested Application FACS, FLISA, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 2B10A8
Isotype IgG2b, kappa
Target Name IgG2b
Antigen Species Rat
Immunogen Rat IgG2b hybridoma
Conjugation FITC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.3 ug/10^6 cells
FLISA1:200 - 1:400
IHC-FrAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse spleen tissue stained with ARG20712 Rat IgG2b Isotype Control antibody [KLH/G2b-1-2] (left) and ARG22104 anti-Ly6G + Ly6C antibody [RB6-8C5] (right) followed by ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) and DAPI.

  • ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) FLISA image

    FLISA: The plate was coated with purified Rat IgG1, IgG2a, IgG2b, IgG2c and IgM. Immunoglobulins were detected with serially diluted ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC).

  • ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20565 anti-CD45 antibody [I3/2.3] followed by ARG21706 Mouse anti-Rat IgG2b antibody [2B10A8] (FITC).