ARG42399

Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE)

Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) for Flow cytometry and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [TB28-2] recognizes Human Kappa Light Chain
Tested Reactivity Hu
Tested Application FACS
Specificity The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
Host Mouse
Clonality Monoclonal
Clone TB28-2
Isotype IgG1, kappa
Target Name Kappa Light Chain
Antigen Species Human
Immunogen Human IgG-kappa myeloma protein.
Conjugation PE

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (4) Click the Picture to Zoom In

  • ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) at 10 µl / 100 µl of peripheral whole blood.

  • ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) FACS image

    Flow Cytometry: Human CD19 positive B cells stained with ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) at 5 µg/ml dilution and co-stained with ARG42396 Mouse anti-Human Lambda Light Chain antibody [1-155-2] (FITC) at 5 µg/ml dilution.

  • ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) FACS image

    Flow Cytometry: Human lymphocytes stained with ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) at 10 µl / 100 µl of peripheral whole blood and co-stained with ARG53782 anti-CD19 antibody [LT19] (APC) at 10 µl / 100 µl of peripheral whole blood.

  • ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) FACS image

    Flow Cytometry: Separation of Human Ig Lambda Kappa Chain positive B cells (red-filled) from Ig Kappa Light Chain negative B cells (black-dashed). Human peripheral whole blood stained with ARG42399 Mouse anti-Human Kappa Light Chain antibody [TB28-2] (PE) at 10 µl / 100 µl of peripheral whole blood.