ARG63035

Mouse anti-Human IgG (Fab) antibody [4A11]

Mouse anti-Human IgG (Fab) antibody [4A11] for ELISA,Flow cytometry,Western blot and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [4A11] recognizes Human IgG (Fab)
Tested Reactivity Hu
Tested Application ELISA, FACS, WB
Specificity The clone 4A11 reacts with Fab fragment of Human IgG.
Host Mouse
Clonality Monoclonal
Clone 4A11
Isotype IgG2b
Target Name IgG (Fab)
Antigen Species Human
Immunogen Purified human IgG.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 5 µg/ml
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Research Area Immune System antibody

Images (2) Click the Picture to Zoom In

  • ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11] WB image

    Western blot: 1) Normal human immunoglobulins, and 2) Normal bovine immunoglobulins stained with ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11].

  • ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11] FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11], followed by incubation with APC-labelled Goat anti-Mouse secondary antibody.