ARG63035
Mouse anti-Human IgG (Fab) antibody [4A11]
Mouse anti-Human IgG (Fab) antibody [4A11] for ELISA,Flow cytometry,Western blot and Human
Immune System antibody
Overview
Product Description | Mouse Monoclonal antibody [4A11] recognizes Human IgG (Fab) |
---|---|
Tested Reactivity | Hu |
Tested Application | ELISA, FACS, WB |
Specificity | The clone 4A11 reacts with Fab fragment of Human IgG. |
Host | Mouse |
Clonality | Monoclonal |
Clone | 4A11 |
Isotype | IgG2b |
Target Name | IgG (Fab) |
Antigen Species | Human |
Immunogen | Purified human IgG. |
Conjugation | Un-conjugated |
Application Instructions
Application Suggestion |
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
---|---|
Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
Preservative | 15 mM Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Background | Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins. |
---|---|
Research Area | Immune System antibody |
Images (2) Click the Picture to Zoom In
-
ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11] WB image
Western blot: 1) Normal human immunoglobulins, and 2) Normal bovine immunoglobulins stained with ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11].
-
ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11] FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG63035 Mouse anti-Human IgG (Fab) antibody [4A11], followed by incubation with APC-labelled Goat anti-Mouse secondary antibody.