ARG82346
Mouse VEGFB ELISA Kit
Mouse VEGFB ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82346-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82346-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82346-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82346-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82346-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82346-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82346-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82346-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82346-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82346-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82346-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82346 Mouse VEGFB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse VEGFB in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | VEGFB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 7.0% |
| Alternate Names | VEGF-related factor; VEGFL; VEGF-B; VRF; Vascular endothelial growth factor B |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49766 Mouse Vascular endothelial growth factor B |
|---|---|
| Gene Symbol | VEGFB |
| Gene Full Name | vascular endothelial growth factor B |
| Background | This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011] |
| Function | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. [UniProt] |
| Cellular Localization | Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | VEGF-B186 is O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82346 Mouse VEGFB ELISA Kit standard curve image
ARG82346 Mouse VEGFB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82346 Mouse VEGFB ELISA Kit User manual |
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