ARG81673
Mouse Tpo / Thrombopoietin ELISA Kit
Mouse Tpo / Thrombopoietin ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81673-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81673-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81673-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81673-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81673-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81673-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81673-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81673-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81673-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81673-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81673-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81673 Mouse Tpo / Thrombopoietin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Tpo / Thrombopoietin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Tpo / Thrombopoietin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5%; Inter-Assay CV: 7.4% |
| Alternate Names | C-mpl ligand; Thrombopoietin; MPLLG; TPO; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; MKCSF; THCYT1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | THPO |
| Gene Full Name | thrombopoietin |
| Background | Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014] |
| Function | Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. [UniProt] |
| Highlight | Related products: Thrombopoietin antibodies; Thrombopoietin ELISA Kits; Thrombopoietin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81673 Mouse Tpo / Thrombopoietin ELISA Kit standard curve image
ARG81673 Mouse Tpo / Thrombopoietin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81673 Mouse Tpo / Thrombopoietin ELISA Kit User's manual |
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