ARG81899

Mouse Tissue Factor Pathway Inhibitor ELISA Kit

Mouse Tissue Factor Pathway Inhibitor ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81899-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81899-002 Standard 2 X 10 ng/vial 4°C
ARG81899-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81899-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81899-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81899-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81899-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81899-008 25X Wash buffer 20 ml 4°C
ARG81899-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81899-010 STOP solution 10 ml (Ready to use) 4°C
ARG81899-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Tissue Factor Pathway Inhibitor in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Tissue Factor Pathway Inhibitor
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%; Inter-Assay CV: 6.5%
Alternate Names TFI; Lipoprotein-associated coagulation inhibitor; LACI; EPI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21788 Mouse TFPI

Swiss-port # O54819 Mouse Tissue factor pathway inhibitor

Gene Symbol TFPI
Gene Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Background This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
Function Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. [UniProt]
Highlight Related products:
Tissue Factor antibodies; Tissue Factor ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM O-glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit standard curve image

    ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81899 Mouse Tissue Factor Pathway Inhibitor ELISA Kit User manual download icon Download